Charge displacement in bacteriorhodopsin during the forward and reverse bR-K phototransition
نویسندگان
چکیده
منابع مشابه
Calculation of proton transfers in Bacteriorhodopsin bR and M intermediates.
Residue ionization states were calculated in nine crystal structures of bacteriorhodopsin trapped in bR, early M, and late M states by multiconformation continuum electrostatics. This combines continuum electrostatics and molecular mechanics, deriving equilibrium distributions of ionization states and polar residue and water positions. The three central cluster groups [retinal Schiff base (SB),...
متن کاملInterpretation of the spatial charge displacements in bacteriorhodopsin in terms of structural changes during the photocycle.
We have recently introduced a method, made possible by an improved orienting technique using a combination of electric and magnetic fields, that allows the three-dimensional detection of the intramolecular charge displacements during the photocycle of bacteriorhodopsin. This method generates electric asymmetry, a prerequisite for the detection of electric signal on the macroscopic sample, in al...
متن کاملBiophysics Infrared evidence that the Schiff base of bacteriorhodopsin is protonated : bR 570 and K intermediates ( purple membrane / resonance
It is possible, by using Fourier-transform infrared (FTIR) difference spectroscopy, to detect the conformational changes occurring in both the protein and the chromophore of bacteriorhodopsin during the photocycle. In contrast to Raman spectroscopy, a laser is unnecessary and hence the problem of a perturbing probe beam is eliminated. Furthermore, the relatively high signal-to-noise ratio obtai...
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PHOTOCYCLE AND PROTON TRANSLOCATION IN BACTERIORHODOPSIN Bacteriorhodopsin (bR), a natural photochromic protein from Halobacterium salinarum (formerly H. halo
This review deals with the role of carboxylic amino acids in the proton transport activity of bacteriorhodopsin. The main focus is on the infrared data, which allow direct monitoring of the protonation/deprotonation of specific residues dur ing the proton movement in the course of the photocycle. Additional attention is paid to the potential use of carboxylic acids in proteins as internal senso...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1995
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(95)80076-7